Patrick CHARNAY
Group leader (DRE Inserm)
INSERM U 784,
Ecole Normale Supérieure
46 rue d’Ulm
75230 Paris Cedex 05,
France
Tel : (33)-1-44-32-36-07
Fax : (33)-1-44-32-39-88
E-Mail
Training and positions :
1973-1976 : Studies of engineering at the Ecole Polytechnique.- 1976-1977 : DEA in Microbiology, University Paris XI.
1977-1981 : PhD thesis in the laboratory of Pierre Tiollais at the Pasteur Institute : Molecular biology of hepatitis B virus.
1981-1984 : Postdoctoral training in the laboratory of Tom Maniatis at Harvard University : Regulation of globin gene expression.
1984-1988 : Group leader at the EMBL : Zinc finger proteins.
1989-1992 : Group leader in the laboratory of Molecular Genetics at the Ecole Normale Supérieure : Hindbrain development.
1993-2005 : Director of the INSERM Unit 368 at the Ecole Normale Supérieure : Nervous system development.
1997- : Associate Professor at the Ecole Polytechnique.
2000-2005 : Director of the Institute of Biology of the Ecole Normale Supérieure (IFR 36) .
2004- : Member of the french Science Academy.
Research topics :
Hindbrain pattern formation
Schwann cell development and myelination in the peripheral nervous system
Development and function of boundary cap cells, putative stem cells of the PNS
Selected recent publications :
Ghislain, J., Desmarquet-Trin-dinh, C., Jaegle, M., Meijer, D., Charnay, P. and Frain, M. Characterisation of cis-acting sequences reveals a biphasic, axon-dependent regulation of Krox20 during Schwann cell development. (2002). Development, 129, 155-166.
Manzanares, M., Nardelli, J., Gilardi-Hebenstreit, P., Marshall, H., Giudicelli, F., Martinez-Pastor, M.T., Krumlauf, R. and Charnay, P. Krox20 and kreisler co-operate in the transcriptional control of Hoxb3 hindbrain segmental expression. (2002). EMBO J., 21, 365-376.
Taillebourg, E., Buart, S. and Charnay, P. A conditional, floxed allele of the Krox20 gene. (2002). Genesis, 32, 112-113.
Zhu, Y., Ghosh, P., Charnay, P., Burns, D.K. and Parada, L.F. Neurofibromas in NF1 : Schwann cell origin and role of tumor environment. (2002). Science, 296, 920-922.
Korsisaari, N., Rossi, D.J., Paetau, A., Charnay, P., Henkemeyer, M. and Mäkelä, T.P. Conditional ablation of the Mat1 subunit of TFIIH in Schwann cells provides evidence that Mat1 is not essential for general transcription (2002). J. Cell Sci., 115, 4275-4284.
Young, P., Boussadia, O., Berger, P., Leone, D., Charnay, P., Kemler, R. and Suter U. E-cadherin is required for the correct formation of autotypic adherens junctions of the outer mesaxon but not for the integrity of myelinated fibers of peripheral nerves (2002). Mol. Cell. Neurosci., 21, 341-351.
Giudicelli, F., Gilardi-Hebenstreit, P., Mechta-Grigoriou, F., Poquet, C. and Charnay, P. Novel activities of Mafb underlie its dual role in hindbrain segmentation and regional specification (2003). Dev. Biol., 253, 150-162.
Ghislain, J., Desmarquet-Trin-Dinh, C., Gilardi-Hebenstreit, P., Charnay, P. and Frain, M. Neural crest patterning : autoregulatory and crest-specific elements co-operate for Krox20 transcriptional control (2003). Development, 130, 941-953.
Vermeren, M., Maro, G., Bron, R., McGonnell, I., Charnay, P., Topilko, P. and Cohen, J. Neural crest boundary cap cells prevent spinal motor neuron emigration at early CNS:PNS interfaces (2003). Neuron, 37, 403-415.
Mechta-Grigoriou, F., Giudicelli, F., Pujades, C., Charnay, P and Yaniv, M. c-jun regulation and function in the developing hindbrain (2003). Dev. Biol., 258, 419-431.
Nardelli, J., Catala, M. and Charnay, P. Establishment of embryonic neuroepithelial cell lines exhibiting an epiplastic expression pattern of region specific markers (2003). J. Neurosci. Res., 73, 737-752.
Young, P., Boussadia, O., Halfter, H., Grose, R., Berger, P., Leone, D., Robenek, H., Charnay, P., Werner, S., Kemler, R. and Suter, U. E-Cadherin controls adherens junctions in the epidermis and the renewal of hair follicles. (2003). EMBO J., 22, 5723-5733.
Garcia-Dominguez, M., Poquet, C., Garel, S. and Charnay, P. Ebf gene function is required for coupling neuronal differentiation and cell cycle exit. (2003). Development, 130, 6013-6025.
Maro, G.S., Vermeren, M., Voiculescu, O., Melton, L., Cohen, J., Charnay, P. and Topilko, P. Neural crest boundary caps constitute a source of neuronal and glial cells of the PNS. (2004). Nature Neurosci, 7, 930-938.
Ghislain, J. and Charnay, P. Control of myelination in Schwann cells : a Krox20 cis-regulatory element integrates Oct6, Brn2 and Sox10 activities. (2006). EMBO Reports, 7, 52-58.
Chomette, D., Frain, M., Cereghini, S., Charnay, P. and Ghislain, J. Krox20 hindbrain cis-regulatory landscape : interplay between multiple long-range initiation and autoregulatory elements. (2006). Development, 133, 1253-1262.
Lopez-Bendito, G., Cautinat, A., Sanchez, J.A., Bielle, F., Flames, N., Charnay, P., Marin, O. and Garel, S. Tangential migration controls axon guidance : a role for neuregulin-1 in thalomocortiocal axon navigation. (2006). Cell,125, 1-16.
Pezeron, G., Anselme, I., Laplante, M., Ellingsen, S., Becker, T.S., Rosa, F.M., Charnay, P., Schneider-Maunoury, S., Mourrain, P. and Ghislain, J. (2006). Duplicate sfrp1 genes in zebrafish : sfrp1a is dynamically expressed in the developing central nervous system, gut and lateral line. (2006) Gene Expression Patterns, Oct ;6(8):835-42.
Garcia-Dominguez, M., Gilardi-Hebenstreit, P. and Charnay, P. PIASxß acts as an activator of Hoxb1 expression and is antagonised by Krox20 during hindbrain segmentation. (2006). EMBO J., Jun 7 ;25(11):2432-42.
Vesque, C., Anselme, I., Couvé, E., Charnay, P. and Schneider-Maunoury, S. Cloning of vertebrate protogenin and comparative expression during axis elongation. (2006) Dev. Dyn., Oct ;235(10):2836-44.
Decker, L., Desmarquet, C., Taillebourg, E., Ghislain, J., Vallat J.-M. and Charnay, P. Myelin maintenance is a dynamic process requiring constant Krox20 expression. (2006) J. Neuroscience, Sep 20 ;26(38):9771-9.
Pouilhe M, Gilardi-Hebenstreit P, Desmarquet-Trin Dinh C and Charnay P. Direct regulation of vHnf1 by retinoic acid signaling and MAF-related factors in the neural tube. (2007). Dev Biol. Sep 15 ;309(2):344-357
Poirier, R., Cheval, H., Mailhes, C., Charnay, P., Davis, S. and Laroche, S. Paradoxical role of an Egr transcription factor family member, Egr2/Krox20, in learning and memory. (2007). /Front. Behav. Neurosci./ *1*:6. doi:10.3389/neuro.08.006.2007.
Poirier, R., Cheval, H., Mailhes, C., Garel, S., Charnay, P., Davis, S. and Laroche, S. Distinct functions of Egr gene family members in cognitive processes. (2008). /Front. Neurosci./ *2*,1:47-55. doi:10.3389/neuro.01.002.2008.
Vázquez-Echeverría, C., Dominguez-Frutos, E., Schimmang, T., Charnay, P. and Pujades, C. Analysis of mouse kreisler mutants reveals new roles of hindbrain-derived FGFs in otic neurogenesis. (2008). Dev. Biol., Oct. 1 ; 322(1):167-178.
Wassef, M., Chomette, D., Pouilhe, M., Stedman, A., Havis, E., Desmarquet-Trin Dinh, C., Schneider-Maunoury, S., Gilardi-Hebenstreit, P., Charnay, P. and Ghislain, J. Rostral hindbrain patterning involves direct activation of a Krox20 transcriptional enhancer by Hox/Pbx and Meis factors. (2008). Development, 135, 3369-3378.
Desmazières, A., Charnay, P. and Gilardi-Hebenstreit, P. Krox20 controls the transcription of its various targets in the developing hindbrain according to multiple modes. (2009). J. Biol. Chem., 284, 10831-10840.
Coulpier, F., Le Crom, S., Maro, G., Manent, J., Giovannini, M., Maciorowski, Z., Fischer, A., Gessler, M., Charnay, P., and Topilko, P. Novel features of boundary cap cells revealed by the analysis of newly identified molecular markers. (2009). Glia, 57, 1450-1457.
Thoby-Brisson, M., McKay, L., Karlen, M., Wu, N., Charnay, P., Champagnat, J. and Fortin, G. Genetic identification of a parafacial oscillator coupling to the preBötzinger complex in the mouse embryo. (2009). Nat. Neurosci., 12, 1028-1035.
Mayer, S.I., Rössler, O.G., Charnay, P. and Thiel, G. Epidermal growth factor-induced proliferation of astrocytes requires Egr transcription factor. (2009). J. Cell Sci., 122, 3340-3350.
Zujovic, V., Thibaud, J., Bachelin, C., Coulpier, F., Charnay, P., Topilko, P. and Baron-Van Evercooren, A. Boundary cap cells are highly competitive for CNS remyelination : fast migration and efficient differentiation in PNS and CNS myelin-forming cells. (2010). Stem cells, 28, 470-479.
Esain, V., Postlethwait, J.H., Charnay, P. and Ghislain, J. FGF-receptor signalling controls neural cell diversity in the zebrafish hindbrain by regulating olig2 and sox9. (2010). Development, 137, 33-42.
Coulpier, F., Decker, L., Vallat, J.-M., Funalot, B., Garcia-Bragado, F., Charnay, P. and Topilko, P. Transgression of the CNS/PNS boundary in the absence of Schwann cells or Egr2/Krox20 function. (2010). J. Neurosci., 30, 5958-5967.
