High Troughput qPCR Facility
High throughput quantitative PCR facility Genomic Paris Centre
Since September 2013, the qPCR-HD-GPC facility offers a quantitative PCR facility broadly available to the scientific community and beyond, to conduct a variety of genomic analyzes such as: absolute quantification, CNV, SNP genotyping, preparation and quantification of genome-banks for targeted sequencing and single cell genomics. The platform is manned by one biological engineer and one platform-dedicated engineer and hosted at the Genomic Platform of the ENS.
The facility is equipped with a Fluidigm Biomark-HDTM system for production of a large number of simultaneous PCR on microfluidic chips (from 2 304 to 36 960), and with 3 microfluidic controllers (AX, MX and HX) enabling one to use most of the available chips.
We offer a service based on a collaboration between the user and the qPCR-HD-GPC facility, that includes experimental design and analysis aimed at delivering publication-ready results. Supported samples include cDNA and genomic DNA and bio-statistical analysis of the results is available under R.
Scientific responsible : Bertrand Ducos (IR, CNRS)
Technical responsible : Marine Delagrange (IE, PSL)
Contact
Previous members :
Juliette Pouch 2013-2016 (AI, ENS) : Technical responsible
Amine Ali Chaouche 2015 (AI, ENS) : Technical responsible
Quentin Viautour 2015-2016 (M2, IE FPGG) : Bioinformatics analyses
Elise Diaz 2016-2017 (AI, ENS) : Technical responsible
Associated Publications :
Heterogeneity of neuroblastoma cell identity defined by transcriptional circuitries.
Impaired PRC2 activity promotes transcriptional instability and favors breast tumorigenesis.
