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Publications

A mechanistic study of helicases with magnetic traps.
Hodeib S, Raj S, Manosas M, Zhang W, Bagchi D, Ducos B, Fiorini F, Kanaan J, Le Hir H, Allemand JF, Bensimon D, Croquette V. (2017)
Protein Science

Mutations in the Spliceosome Component CWC27 Cause Retinal Degeneration with or without Additional Developmental Anomalies.
Xu M, Xie YA, Abouzeid H, et al. (2017)
American Journal of Human Genetics

Insights into the design and interpretation of iCLIP experiments.
Haberman N, Huppertz I, Attig J, König J, Wang Z, Hauer C, Hentze MW, Kulozik AE, Le Hir H, Curk T, Sibley CR, Zarnack K, Ule J. (2017)
Genome Biology

The exon junction complex as a node of postranscriptional networks.
Le Hir H, Saulière J, Wang Z. (2016)
Nature Review in Molecular and Cellular Biology. Jan ;17(1):41-54.

Human Upf1 is a highly processive RNA helicase and translocase with RNP remodelling activities.
Fiorini F, Bagchi D, Le Hir H*, Croquette V*. (2015)
Nature Communications. Jul 3 ;6:7581.
*Corresponding authors

CLIP-seq to discover transcriptome-wide imprinting of RNA binding proteins in living cells.
Saulière J, Le Hir H. (2015)
Methods Molecular Biology. 1296:151-60.

Transcriptome-wide modulation of splicing by the Exon Junction Complex.
Wang Z, Murigneux V, Le Hir H. (2014)
Genome Biol. Dec 5 ;15(12):551.

Crystal structure of the human eIF4AIII-CWC22 complex shows how a DEAD-box protein is inhibited by a MIF4G domain.
Buchwald G, Schüssler S, Basquin C, Le Hir H, Conti E.
Proc Natl Acad Sci U S A. 2013 Nov 26 ;110(48)

Transcriptome-wide identification of RNA binding sites by CLIP-seq.
Murigneux V, Saulière J, Roest Crollius H, Le Hir H. (2013)
Methods

EJC core component MLN51 interacts with eIF3 and activates translation.
Chazal PE, Daguenet E, Wendling C, Ulryck N, Tomasetto C, Sargueil B, Le Hir H. (2013)
Proc Natl Acad Sci U S A

Tight intramolecular regulation of the human Upf1 helicase by its N- and C-terminal domains.
Fiorini F, Boudvillain M, Le Hir H. (2013)
Nucleic Acids Res.

CLIP-seq of eIF4AIII reveals transcriptome-wide mapping of the human exon junction complex.
Saulière J, Murigneux V, Wang Z, Marquenet E, Barbosa I, Le Tonquèze O, Audic Y, Paillard L, Roest Crollius H, Le Hir H. (2012)
Nature structural & molecular biology

Human CWC22 escorts the helicase eIF4AIII to spliceosomes and promotes exon junction complex assembly.
Barbosa I, Haque N, Fiorini F, Barrandon C, Tomasetto C, Blanchette M, Le Hir H. (2012)
Nature structural & molecular biology

The human T-lymphotropic virus type 1 tax protein inhibits nonsense-mediated mRNA decay by interacting with INT6/EIF3E and UPF1.
Mocquet V, Neusiedler J, Rende F, Cluet D, Robin JP, Terme JM, Duc Dodon M, Wittmann J, Morris C, Le Hir H, Ciminale V, Jalinot P. (2012)
J Virol.

Perispeckles are major assembly sites for the exon junction core complex.
Daguenet E, Baguet A, Degot S, Schmidt U, Alpy F, Wendling C, Spiegelhalter C, Kessler P, Rio MC, Le Hir H, Bertrand E, Tomasetto C. (2012)
Mol Biol Cell.

Proteins associated with the exon junction complex also control the alternative splicing of apoptotic regulators.
Michelle L, Cloutier A, Toutant J, Shkreta L, Thibault P, Durand M, Garneau D, Gendron D, Lapointe E, Couture S, Le Hir H, Klinck R, Elela SA, Prinos P, Chabot B. (2012)
Mol Cell Biol.

Biochemical characterization of the RNA helicase UPF1 involved in nonsense-mediated mRNA decay.
Fiorini F, Bonneau F, Le Hir H. (2012)
Methods in Enzymology

Molecular mechanisms for the RNA-dependent ATPase activity of Upf1 and its regulation by Upf2.
Chakrabarti S, Jayachandran U, Bonneau F, Fiorini F, Basquin C, Domcke S, Le Hir H, Conti E (2012)
Mol Cell

The structure of the ASAP core complex reveals the existence of a Pinin-containing PSAP complex.
Murachelli AG, Ebert J, Basquin C, Le Hir H, Conti E (2011)
Nature structural & molecular biology

The exon junction complex differentially marks spliced junctions.
Saulière J, Haque N, Harms S, Barbosa I, Blanchette M, Le Hir H, (2010)
Nature structural & molecular biology

Insights into the recruitment of the NMD machinery from the crystal structure of a core EJC-UPF3b complex.
Buchwald G, Ebert J, Basquin C, Sauliere J, Jayachandran U, Bono F, Le Hir H, Conti E, (2010)
Proceedings of the National Academy of Sciences of the United States of America

A UPF3-mediated regulatory switch that maintains RNA surveillance.
Chan WK, Bhalla AD, Le Hir H, Nguyen LS, Huang L, Gécz J, Wilkinson MF, (2009)
Nature structural & molecular biology

Mechanism of ATP turnover inhibition in the EJC.
Nielsen KH, Chamieh H, Andersen CB, Fredslund F, Hamborg K, Le Hir H, Andersen GR, (2009)
RNA (New York, N.Y.)

EJCs at the heart of translational control.
Le Hir H, Séraphin B, (2008)
Cell

Structural insights into the exon junction complex.
Le Hir H, Andersen GR, (2008)
Current opinion in structural biology

NMD factors UPF2 and UPF3 bridge UPF1 to the exon junction complex and stimulate its RNA helicase activity.
Chamieh H, Ballut L, Bonneau F, Le Hir H, (2008)
Nature structural & molecular biology

The exon-junction-complex-component metastatic lymph node 51 functions in stress-granule assembly.
Baguet A, Degot S, Cougot N, Bertrand E, Chenard MP, Wendling C, Kessler P, Le Hir H, Rio MC, Tomasetto C, (2007)
Journal of cell science

Identification and characterization of human Mex-3 proteins, a novel family of evolutionarily conserved RNA-binding proteins differentially localized to processing bodies.
Buchet-Poyau K, Courchet J, Le Hir H, Séraphin B, Scoazec JY, Duret L, Domon-Dell C, Freund JN, Billaud M, (2007)
Nucleic acids research

Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA.
Andersen CB, Ballut L, Johansen JS, Chamieh H, Nielsen KH, Oliveira CL, Pedersen JS, Séraphin B, Le Hir H, Andersen GR, (2006)
Science (New York, N.Y.)

The exon junction core complex is locked onto RNA by inhibition of eIF4AIII ATPase activity.
Ballut L, Marchadier B, Baguet A, Tomasetto C, Séraphin B, Le Hir H, (2005)
Nature structural & molecular biology

Expression of GFRalpha1 receptor splicing variants with different biochemical properties is modulated during kidney development.
Charlet-Berguerand N, Le Hir H, Incoronato M, di Porzio U, Yu Y, Jing S, de Franciscis V, Thermes C, (2004)
Cellular signalling

Association of the breast cancer protein MLN51 with the exon junction complex via its speckle localizer and RNA binding module.
Degot S, Le Hir H, Alpy F, Kedinger V, Stoll I, Wendling C, Seraphin B, Rio MC, Tomasetto C, (2004)
The Journal of biological chemistry

Splicing enhances translation in mammalian cells : an additional function of the exon junction complex.
Nott A, Le Hir H, Moore MJ, (2004)
Genes & development

DcpS can act in the 5 -3 mRNA decay pathway in addition to the 3 -5 pathway.
van Dijk E, Le Hir H, Séraphin B, (2003)
Proceedings of the National Academy of Sciences of the United States of America

How introns influence and enhance eukaryotic gene expression.
Le Hir H, Nott A, Moore MJ, (2003)
Trends in biochemical sciences

5 exon interactions within the human spliceosome establish a framework for exon junction complex structure and assembly.
Reichert VL, Le Hir H, Jurica MS, Moore MJ, (2002)
Genes & development

5 -End RET splicing : absence of variants in normal tissues and intron retention in pheochromocytomas.
Le Hir H, Charlet-Berguerand N, de Franciscis V, Thermes C, (2002)
Oncology

The protein Mago provides a link between splicing and mRNA localization.
Le Hir H, Gatfield D, Braun IC, Forler D, Izaurralde E, (2001)
EMBO reports

The DExH/D box protein HEL/UAP56 is essential for mRNA nuclear export in Drosophila.
Gatfield D, Le Hir H, Schmitt C, Braun IC, Köcher T, Wilm M, Izaurralde E, (2001)
Current biology : CB

The exon-exon junction complex provides a binding platform for factors involved in mRNA export and nonsense-mediated mRNA decay.
Le Hir H, Gatfield D, Izaurralde E, Moore MJ, (2001)
The EMBO journal

The spliceosome deposits multiple proteins 20-24 nucleotides upstream of mRNA exon-exon junctions.
Le Hir H, Izaurralde E, Maquat LE, Moore MJ, (2001)
The EMBO journal

Relative expression of the RET9 and RET51 isoforms in human pheochromocytomas.
Le Hir H, Charlet-Berguerand N, Gimenez-Roqueplo A, Mannelli M, Plouin P, de Franciscis V, Thermes C, (2000)
Oncology

Pre-mRNA splicing alters mRNP composition : evidence for stable association of proteins at exon-exon junctions.
Le Hir H, Moore MJ, Maquat LE, (2000)
Genes & development