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Karthala AODscope multiphoton

Detection :

Dodt contraste with video rate
4 PMT GaAsP photon counting or analog (2 epifluorescence and 2transfluorescence)
Light guide epi detection
High efficiency design detection

Scanning :

Two AOD tunable design with temporal and spatial compensation laser lines ( 700-900nm and 800-1000nm). they can work simultaneously or separate.

Mode Random-Access scanning :
This allows to increase the dwell-time per point and dramatically improves the resolution. 50Khz/nbr point .( Otsu et al. paper Neuron 2014)

Mode fast image scanning :
Capacity to scan at adjustable speed from 0.1µs/pixel (resonant speed) to any dwell time, this spanning the range between resonant scanners and classical scanners. Contrary to resonant scanners the scan is linear and sub-fields can be scanned at resonant speed’s also possible to make multi region of interest.The field of view is 350 µm.

Stabilisation of sample :
Automatic 3D stabilisation of episodic acquisitions (during the inter-episode dwell time, ie in between 2 repetitive stimulations for example)

DIMM software :

lab-proof user interface using labview

Associate Paper :

Ultrafast random-access scanning in twophoton microscopy using acousto-optic deflectors.Salome´ R et al (2006) J Neurosci Methods

A spatio-temporally compensated acousto-optic scanner for two-photon microscopy providing large field of view. Kremer Y et al Opt Express 2008

Optical monitoring of neuronal activity at high frame rate with a digital random-access multiphoton (RAMP).Otsu Y et al (2008) J Neurosci Methods

Activity-Dependent Gating of Calcium Spikes by A-type K+ Channels ControlsClimbing fiber signaling in purkinje cell dentrires. Ostsu Y et al , Neuron (2014)

Interplay between Synchronization of Multivesicular Release and Recruitmentof Additional Release Sites Support Short-Term Facilitation at Hippocampal Mossy
Fiber to CA3 Pyramidal Cells Synapses .
Chamberland et al Jneurosci 2014