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Protein Engineering Facility

Head of the facility: David Stroebel

News 01/2022 : Presentation of the facility : IBENS exchange day CodEq-Facilities the 31/01/2022.

The Protein Engineering Facility of IBENS manages a collection of equipments related to protein over-expression, cell lysis, purification and analysis.

Local users can have access to the equipments and David Stroebel, head of the facility, will be glad to give you some help and advises on any of your biochemistry-related projets.

IBENS users have free access to the following equipments :
 Imager LAS-4000 for chimiluminescence imaging (on schedulled).
 Cell Breakers : French Press and Cell D system
 Spectrophotometer and fluorimeter cuvette (JASCO) and plate (TECAN).
 Ultracentrifuge TL-100.

For your safety and for the machine well-being, please note that any first use of a machine MUST be done in the presence of the head of the facility (please contact him first).

Other equipments can be used only under the direct supervision of the head of the facility or under collaboration.

 Protein purification can be achieved using the FPLC placed in a refrigerated compartment. SEC columns for mass analysis can be borrowed on demand. An online fluorimeter detector is available.

 Baculovirus equipments. A full set of equipments is available for protein production using the baculovirus expression system (incubators, hood, binocular).

 Isothermal Calorimetry (ITC 200). A powerfull machine allowing the precise characterisation of the interaction parameters between two molecular partners in vitro . We can make experiments and analysis on demand (see publications below). This machine is used almost exclusively on collaboration. The form to use the machine (with an explanation of the experimental conditions) is accessible on the top right of this page (itc_experiments_form_2017_eng.doc).

Many other equipments of in vitro biophysical analysis of biomolecules are accessible on demand to David Stroebel through the local network of biophysics equipment of the Montagne Sainte Geneviève.

Publications involving the facility:

2021 Eur Biophys J. Velours C, Aumont-Nicaise M, Uebel S, England P, Velazquez-Campoy A, Stroebel D, Bec G, Soule P, Quétard C, Ebel C., Roussel A, Charbonnier J-B, Fernández Varela P. ’Macromolecular interactions in vitro, comparing classical and novel approaches’. 50(3-4):313-330.

2018 Elife. Nassrallah A, Rougée M, Bourbousse C, Drevensek S, Fonseca S, Iniesto E, Ait-Mohamed O, Deton-Cabanillas AF, Zabulon G, Ahmed I, Stroebel D, Masson V, Lombard B, Eeckhout D, Gevaert K, Loew D, Genovesio A, Breyton C, De Jaeger G, Bowler C, Rubio V, Barneche F. ’DET1-mediated degradation of a SAGA-like deubiquitination module controls H2Bub homeostasis’. Sep 7;7. pii: e37892. doi: 10.7554/eLife.37892

2017 Nat Commun. Yu IM, Planelles-Herrero VJ, Sourigues Y, Moussaoui D, Sirkia H, Kikuti C, Stroebel D, Titus MA, Houdusse A. ‘Myosin 7 and its adaptors link cadherins to actin.’ ; 29;8:15864.

2017 Nat Commun. Frémont S, Hammich H, Bai J, Wioland H, Klinkert K, Rocancourt M, Kikuti C, Stroebel D, Romet-Lemonne G, Pylypenko O, Houdusse A, Echard A. ‘Oxidation of F-actin controls the terminal steps of cytokinesis’ ; 23;8:14528.

2016 Neuron. Fossati M, Pizzarelli R, Schmidt ER, Kupferman JV, Stroebel D, Polleux F, Charrier C. ‘SRGAP2 and its Human-Specific Paralog Co-regulate the Development of Excitatory and Inhibitory Synapses.’ ; 91(2):356-69